咨詢： （重慶） （貴陽） Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody 抗泛素抗體產(chǎn)品信息： Product name：Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibodySee all Ubiquitin primary antibodies Description Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63) Tested applicationsWB, IHC-P, ICC/IF more details Species reactivity Reacts with: Mouse, Rat, Human Positive control K63-linked-Ub2-7 recombinant protein; Human kidney carcinoma tissue. Properties FormLiquid Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. PurityTissue culture supernatant ClonalityMonoclonal Clone numberEPR8590-448 IsotypeIgG Research Areas Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin Neuroscience Neurology process Neurodegenerative disease Other Epigenetics and Nuclear Signaling Ubiquitin & Ubiquitin Like Modifiers Ubiquitin like Modifiers Applications Our Abpromise guarantee covers the use of ab179434 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Notes WB WB: 1/1000 - 1/5000. Predicted molecular weight: 77 kDa. IHC-P IHC-P: 1/100 - 1/250. ICC/IF ICC/IF: 1/100 - 1/250. Application notesIs unsuitable for ,Flow Cyt or IP. Target RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains. Cellular localizationCell Membrane, Cytoplasmic and Nuclear Database links Entrez Gene: 7314 Human Entrez Gene: 22187 Mouse Entrez Gene: 192255 Rat Omim: 191339 Human SwissProt: P0CG47 Human SwissProt: P0CG48 Human SwissProt: P62988 Human SwissProt: P0CG49 Mouse Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody images Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434) Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with ab179434 at 1/100 dilution. Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434) Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434) All lanes : Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody (ab179434) at 1/1000 dilution Lane 1 : Ubiquitin recombinant protein Lane 2 : K6-linked-Ub2 recombinant protein Lane 3 : K11-linked-Ub2 recombinant protein Lane 4 : K27-linked-Ub2 recombinant protein Lane 5 : K29-linked-Ub2 recombinant protein Lane 6 : K33-linked-Ub2 recombinant protein Lane 7 : K48-linked-Ub2-7 recombinant protein Lane 8 : K63-linked-Ub2-7 recombinant protein Lysates/proteins at 0.02 µg per lane. Predicted band size : 77 kDa 公司介紹： Abcam位于英國(guó)的劍橋科學(xué)園，成立于1998年，專門生產(chǎn)和分銷研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司，理念是Abcam銷售質(zhì)量高的抗體，并提供zui全面、誠(chéng)實(shí)、時(shí)新的說明書，快速的送貨服務(wù)，及高效率的顧客和服務(wù)。我們的在線目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑，并不斷添加，供應(yīng)予百多個(gè)國(guó)家。Abcam于2005年11月在倫敦證券交易所上市，在美國(guó)馬薩諸塞州、日本東京及香港均設(shè)有分公司。 Abcam 一直致力加強(qiáng)產(chǎn)品線，為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品，在2011并購(gòu)了美國(guó)的MitoSciences公司，加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng)；同年也并購(gòu)了英國(guó)的Ascent Scientific 公司，開展了生化試劑的供應(yīng)。在2012年，Abcam并購(gòu)了美國(guó)的Epitomics 公司，成為一家有的RabMAbs® 供應(yīng)商。 Abcam 的目標(biāo)是給世界上的抗體建立zui大的在線目錄，為各地科學(xué)家提供*產(chǎn)品，成為各國(guó)科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來使客戶獲得預(yù)期的結(jié)果。為提供高質(zhì)量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠(chéng)信才是上策，在我們的上會(huì)發(fā)布有關(guān)我們每一種產(chǎn)品的盡可能多的信息。 更多產(chǎn)品： Abcamab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptor抗體一抗 Abcamab11320Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗 Abcamab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulin抗體一抗 Abcamab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulin抗體一抗 Abcamab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulin抗體一抗 Abcamab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulin抗體一抗 Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗 Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗體一抗 Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗體一抗 Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗體一抗 Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗體一抗 Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗體一抗 更多詳情，請(qǐng)： 重慶市華雅干細(xì)胞技術(shù)有限公司 |
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